The Ultimate Guide To Understanding HPLC Vials

HPLC chromatography is used in analytical chemistry, biochemistry, and most laboratories globally. It splits compound mixtures to identify, quantify, and purify the specific components of the mixture.

But did you know choosing the wrong HPLC vials can ruin your analysis and degrade your samples? Do you know how to choose the right vials for your testing?

The following guide will explain the importance of selecting the correct vials for your HPLC machine and how it’s done. This will ensure accurate results of your HPLC test.

Auto-Sampler Compatibility

Some auto-samplers use robotic arms to pick up vials and some rotate the tray to line up the sampling needle with the vial.

These samplers require specific vial sizes and types of closure for proper function. The wrong type of vial could result in damages to the machine.

HPLC Column and Closure

Selecting the right type of closures for your vials will depend on your auto-sampler. It’s important to analyze which type is correct for your equipment.

Screw tops are reusable and don’t require any special tools for application.

Crimp tops need aluminum seals and are not able to be reused. However, they provide the best long-term seal sample storage.

Snap rings don’t have seals that are as secure as screw tops or crimp tops. Their advantage is that they’re less prone to breaking when decapping.

Headspace closures are available in both screw top and crimp top options. Also, shell closures are a cheaper option to screw tops.

Types of Vials and Caps

Selecting the right types of HPLC vials and caps is crucial.

The inertness and cleanliness of your vials are key. It’s important to reduce contaminants to avoid ruining the sample. The products you buy should have a quality certification and validate their production process.

Make sure the vial manufacturer has cleanroom production policies and uses proper quality control measures.

Cap selection is also crucial to stopping contamination and preventing damage to the needle. Types of caps include screw top, short thread, snap ring, push-on, crimp top, and headspace.

Caps are usually made from aluminum for crimp top vial options. They’re made from plastic for all non-crimp top vial options. Septa linings with PTFE lamination are sold separately for insertion into the caps.

Other Factors To Consider

Determine the size of your sample before purchasing your vials. Common sizes include 1ml, 2ml, and 4ml.

Consider the vial material. Glass is more heat resistant than plastic and better for testing PH-sensitive samples. Plastic vials provide higher chemical resistance for samples sensitive to sticking on glass.

Choose the correct color. Amber vials are better for samples sensitive to UV light. Clear vials are fine anytime light sensitivity isn’t an issue for your samples.

Choose Your HPLC Vials With Confidence

Now you know what factors go into selecting the correct HPLC vials for your testing. Your samples will remain safe and your analysis will be unobstructed. Choose your vials with confidence!

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